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fluorospot plus mouse ifn  (Mabtech Inc)

 
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    Mabtech Inc fluorospot plus mouse ifn
    Fluorospot Plus Mouse Ifn, supplied by Mabtech Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluorospot+plus+mouse+ifn/pm42172905-68-30-35?v=Mabtech+Inc
    Average 86 stars, based on 1 article reviews
    fluorospot plus mouse ifn - by Bioz Stars, 2026-08
    86/100 stars

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    Cellular responses. Splenocytes from immunized mice (1 mouse/group) were collected and stimulated either without (none), with pooled peptides of S (S peptides) or with Con A followed by detection of <t>IFN-γ</t> <t>or</t> <t>IL-4</t> cytokines in triplicate using FluoroSpot assay. (A) FluoroSpot wells show the IFN-γ or IL-4 released/5 × 10 5 cells. (B) Spot-forming cells (SFC) from (A) were quantitated and calculated. Empty and slash columns indicate none and S peptides stimulations, respectively. The data are presented as mean ± SEM, and the statistical significance between the groups under peptide stimulation was analyzed using Student's t -test (* p < 0.05). (C) Stratified SFC ratios of IFN-γ (Th1) vs. IL-4 (Th2).
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    Cellular responses. Splenocytes from immunized mice (1 mouse/group) were collected and stimulated either without (none), with pooled peptides of S (S peptides) or with Con A followed by detection of IFN-γ or IL-4 cytokines in triplicate using FluoroSpot assay. (A) FluoroSpot wells show the IFN-γ or IL-4 released/5 × 10 5 cells. (B) Spot-forming cells (SFC) from (A) were quantitated and calculated. Empty and slash columns indicate none and S peptides stimulations, respectively. The data are presented as mean ± SEM, and the statistical significance between the groups under peptide stimulation was analyzed using Student's t -test (* p < 0.05). (C) Stratified SFC ratios of IFN-γ (Th1) vs. IL-4 (Th2).

    Journal: International Journal of Biological Macromolecules

    Article Title: Nanoparticular CpG-adjuvanted SARS-CoV-2 S1 protein elicits broadly neutralizing and Th1-biased immunoreactivity in mice

    doi: 10.1016/j.ijbiomac.2021.11.020

    Figure Lengend Snippet: Cellular responses. Splenocytes from immunized mice (1 mouse/group) were collected and stimulated either without (none), with pooled peptides of S (S peptides) or with Con A followed by detection of IFN-γ or IL-4 cytokines in triplicate using FluoroSpot assay. (A) FluoroSpot wells show the IFN-γ or IL-4 released/5 × 10 5 cells. (B) Spot-forming cells (SFC) from (A) were quantitated and calculated. Empty and slash columns indicate none and S peptides stimulations, respectively. The data are presented as mean ± SEM, and the statistical significance between the groups under peptide stimulation was analyzed using Student's t -test (* p < 0.05). (C) Stratified SFC ratios of IFN-γ (Th1) vs. IL-4 (Th2).

    Article Snippet: Cellular immunity assay was performed using the Mouse IFN-γ/IL-4 FluoroSpot PLUS kit (Mabtech).

    Techniques: Flurospot